Articles written by Subhankar Chakraborty

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About me - Subhankar Chakraborty

I am a graduate student in the department of Biochemistry. i completed my bachelors degree in medicine from india and currently work on pancreatic cancer.

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Sciences > Molecular Biology Molecular biology techniques: RNA interference: 2 of 4
RNA interferenceRNA interference is the phenomenon wherein a double stranded RNA (ds RNA) molecule with two overhanging nucleotides (usually TT or UU) at its 3' end (called a "short interfering RNA" or siRNA) silences the expression of a gene at the post-transcription level. siRNA molecules need to be differentiated from anti... More..
Sciences > Molecular Biology DNA transcription: Searching for the source of gene expression: 1 of 2
H-DNA as a Potential cis-Element Regulating Gene Expression Subhankar ChakrabortyDNA triplexes are formed when a polypurine segment of duplex DNA is recognized and bound by a single stranded oligonucleotide lodging in the major groove of the duplex. Such a single stranded oligonucleotide is termed as a triplex-forming oligonu... More..
Sciences > Molecular Biology mRNA stability: A mechanism for regulating gene expression: 1 of 1
IntroductionEvery fully processed mRNA in eukaryotic cells has a poly (A) tail at its 3'end. This stretch of polyadenine residues serves several functions including increasing mRNA stability, enhancing its translation and regulating the transport of fully processed mRNA into the cytoplasm(Colgan and Manley, 1997). In vitro, u... More..
Sciences > Biology (Other) Science behind low carb diet plans: 1 of 1
Carbohydrate Response Element binding Protein (ChREBP) and its role in glucose activated transcription of Lipogenic enzymes Excess dietary carbohydrate is converted in the liver into triglycerides. Following a carbohydrate rich diet, several enzymes catalyzing glycolysis and lipogenesis are activated including L-type Pyruvate... More..
Sciences > Molecular Biology Molecular biology techniques: Comparative genomic hybridization: 1 of 1
COMPARATIVE GENOMIC HYBRIDIZATION (CGH)Comparative genomic hybridization is a technique to study changes in copy number of DNA. Classical CGH procedure consists in labeling DNA from the "test" patient" with a particular fluorescent dye that emits green light, while DNA from a normal individual serves as the control and is lab... More..
Sciences > Molecular Biology Repeating elements in the human genome: Role in evolution: 1 of 1
Simple sequence repeats (SSR)SSRs comprise two groups of repeat elements, micro and macro/minisatellites. SSRs are defined as tandemly repeated short motifs. Microsatellite repeats typically have between 1 upto 6 bases in each repeat, while macrosatellites have a minimum of 5-6 and upwards ranging upto several dozen per repea... More..
Sciences > Molecular Biology Molecular biology techniques: Subtractive hybridization: 1 of 1
Subtractive hybridizationSubtractive hybridization (SH) is a technique which is used to determine the difference between two genomes, i.e. if two DNA samples are otherwise identical except for a region that has been deleted (as is common in several cancers) or added (as in case of infectious organisms whose genome might be ad... More..
Sciences > Anatomy Mucins explained: 1 of 1
Definition:Mucins are high molecular weight glycoproteins that serve the functions of protection and lubrication in the epithelium lined ducts and lumens in the human bodyMucins are present at the interface of several secretory and excretory surfaces with the external and internal environment. These surfaces experience contin... More..
Health & Fitness > Cancer Explaining pancreatic cancer: 9 of 9
P16 mutations in pancreatic cancer (PC)p16 protein inhibits the activity of the cyclin D/CDK-1 complex which phosporylates retinoblastoma protein (Rb), a step necessary for the cell to cross the G1 checkpoint. Mutation of p16 results in uncontrolled phosphorylation of Rb protein, in turn resulting in uncontrolled cell prolife... More..
Sciences > Molecular Biology Mitochondrial DNA and human evolution: In search of Eve: 2 of 2
Mitochondrial DNA and human evolutionMitochondrial DNA is an attractive target to study human evolution for several reasons:- it is present in numerous copies(several thousand copies exist compared to only two copies of the nuclear genome)- it does not undergo recombination (although new evidence suggests that it may recombin... More..