by Luana

Created on: December 12, 2009

What  is a  dilution?

A dilution is a reduction in the concentration of a solution. This will include a stock solution (solution to be reduced) and a Diluent (reducing agent eg distilled water).

To calculate the new concentration of a diluted solution one  must KNOW

 -  the Concentration of the  Original or Stock solution { C1 },

-  the Volume of the Stock solution{ V1 }  ,

-  the Volume of the Final solution {V2}.

 First have to CALCULATE the V1     since the C1 and  V2  are usually known

For example prepare 5ml of a 1:5 dilution from a stock of  10mg/ml

 V1  = Volume of Final solution  X  Dilution factor

      =  5ML                                  X  1/5

     =1ML OF STOCK SOL. To be used to make dilution.

  Since  you KNOW the Concentration of the  Original or Stock solution { C1 }, the Volume of the Stock solution{ V1 }  and the Volume of the Final solution {V2}.

This formula is used C1  V1 =  C2  V2

                                          C2  =  C1  V1          = 10mg/ml  x 1ml  =      2mg/ml

                                                       V2                         5ml    

Therefore the concentration of 5ml  of 1:5  dilution is 2mg/ml..                                                                                   

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